PRP-X100洗脫液濃縮液

更新時間：2018-07-06

點擊次數：2762

Hamilton Company專注于精密測量設備，自動化液體處理工作站和樣品管理系統的開發，制造和定制。Hamilton的流程針對質量和靈活性進行了優化。無論是具有快速交付時間框架的定制針頭，特殊長度的pH傳感器，還是全面自動化您的檢測工作流程的全面解決方案，相信Hamilton產品將始終滿足您的需求。

Hamilton公司60多年來一直是的制造商，總部位于內華達州里諾市; 馬薩諸塞州富蘭克林; 蒂米什瓦拉，羅馬尼亞; 和瑞士的Bonaduz; 和遍布的子公司。

PRP-X100洗脫液濃縮液

PRP-X100 Eluent Concentrate

Hamilton為PRP-X100陰離子交換HPLC色譜柱提供了一種快速簡便的方法來制備4.0 mM對羥基苯甲酸/ 2.5％甲醇，pH 8.5流動相。

為PRP-X100陰離子交換柱快速制備流動相
沒有計算，只需混合并運行樣品

零件號/ REF	描述
79325	Eluent Concentrate，PRP-X100陰離子交換，（60 mL瓶裝，每瓶1瓶）
79335	Eluent Concentrate，PRP-X100陰離子交換，（60 mL瓶裝，每瓶6瓶）

HPLC樹脂規格

Reversed Phase Columns

Packing Name	Support Material	Exchange Capacity	Pore Size	Temp. Limits	Phase Limits	Buffer Strength	Maximum Pressure
PRP-1	PSDVB*	N/A	100 Å	5-85 °C	pH 1-13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi
PRP-C18	C18 bonded to PSDVB*	N/A	100 Å	5-85 °C	pH 1 – 13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi
PRP-3	PSDVB*	N/A	300 Å	5-85 °C	pH 1-13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi
PRP-h5	Fluorinated PSDVB*	N/A	300 Å	5-85 °C	pH 1 – 13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi
HxSil C18	Silica	N/A	110 Å	5-60 °C	pH 1.5 – 10. 0-100% aqueous, organic modifier	N/A	6,000 psi
HxSil C8	Silica	N/A	110 Å	5-60 °C	pH 1.5 – 10. 0-100% aqueous, organic modifier	N/A	6,000 psi

Anion Exchange Columns

Packing Name	Support Material	Exchange Capacity	Pore Size	Temp. Limits	Phase Limits	Buffer Strength	Maximum Pressure
PRP-X100	PSDVB* with Tri- methyl ammonium Exchanger	0.19meq/gm	100 Å	pH Dependent**	pH 1-13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi
PRP-X110	PSDVB* with Tri-methyl ammonium Exchanger	0.11meq/gm	100 Å	pH Dependent**	pH 1-13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi
PRP-X500	Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride)	1.6meq/gm	Superficially porous	pH Dependent**	pH 1-13. 0-100% aqueous, organic modifier	0.0-2.0N	5,000 psi
PRP-X600	Poly (di-methyl amido-propyl meth-acrylamide)	1.6meq/gm	Superficially porous	pH Dependent**	pH 1-13. 0-100% aqueous, organic modifier	0.0-1.0N	5,000 psi
RCX-10	PSDVB* with Tri-methyl ammonium Exchanger	0.35meq/gm	100 Å	pH Dependent**	pH 1-13. 0-100% aqueous, organic modifier	0.0-1.0N	5,000 psi
RCX-30	PSDVB* with Tri-methyl ammonium Exchanger	1.0meq/gm	100 Å	pH Dependent**	pH 1-13. 0-100% aqueous, organic modifier	0.0-1.0N	5,000 psi

Cation Exchange Columns

Packing Name	Support Material	Exchange Capacity	Pore Size	Temp. Limits	Phase Limits	Buffer Strength	Maximum Pressure
PRP-X200	PSDVB* Sulfonate Exchanger	35ueq/gm	100 Å	5-60 °C	pH 1-13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi
PRP-X400	PSDVB* Sulfonate Exchanger	2.5meq/gm	N/A	5-60 °C	pH 1-13. 0-100% aqueous, organic modifier	0.0-5.0N	5,000 psi
PRP-X800	PSDVB* Itaconate Exchanger	1.6meq/gm	100 Å	5-60 °C	pH 1-13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi
HC-40 Calcium	PSDVB* Sulfonate Exchanger	5meq/gm	Gel-type	25-90 °C	100% Water	Water	1,000 psi
HC-75 Calcium	PSDVB* Sulfonate Exchanger	5meq/gm	Gel-type	25-90 °C	100% Water 0-40% Acetonitrile	Water	400 psi
HC-75 Hydrogen	PSDVB* Sulfonate Exchanger	5meq/gm	Gel-type	25-90 °C	100% Water 0-40% Acetonitrile	Water	400 psi
HC-75 Lead	PSDVB* Sulfonate Exchanger	5meq/gm	Gel-type	25-90 °C	100% Water 0-40% Acetonitrile	Water	400 psi

Ion Exclusion Columns

Packing Name	Support Material	Exchange Capacity	Pore Size	Temp. Limits	Phase Limits	Buffer Strength	Maximum Pressure
PRP-X300	PSDVB* Sulfonate Exchanger	0.17meq/gm	100 Å	5-60 °C	pH 1-13. 0-100% aqueous, organic modifier	0.0-0.5N	5,000 psi

*PSDVB is Poly(styrene-divinylbenzene)
**pH 1-7.9 Temp. 5-60 °C; pH 8-13 Temp. 5-30 °C

HPLC色譜柱保養和儲存

HPLC柱具有有限的壽命。建議您使用標準定期監測色譜柱的保留特性和性能。如果您打算長時間儲存??色譜柱，請使用抑制微生物生長的流動相（含有sodium azide或高濃度甲醇，乙腈等的流動相）。為獲得適性能和色譜柱壽命，請遵循以下建議。

定期監控色譜柱的性能。
僅在可混溶的流動相之間切換。
避免在色譜柱中沉淀鹽。
使用過濾和脫氣的流動相。
不要讓色譜柱變干。不使用時請將其蓋緊。
對于長期儲存，使用抑制細菌和霉菌生長的流動相。
按標簽上指示的方向使用該列。異常高的操作壓力可能表明插入的入口玻璃料堵塞，并且可以通過使流過柱的流量反轉10-20mL來清除。
使用Hamilton保護柱去除可能終身性結合分析柱的顆粒物質或雜質。

恢復色譜柱性能
下面列出的步驟可能會恢復已經污染的色譜柱的性能，但這些過程不會改善損壞或超出其使用壽命的色譜柱的性能。也不能恢復所有固定相。PRP-X500和PRP-X600色譜柱的*支持使得無法通過洗滌程序恢復性能。有關這些色譜柱的技術幫助，請HPLC支持。注意：在執行恢復程序之前，請務必確保流動相是可混溶的，并且不會發生沉淀。如有必要，在轉換到新的流動相之前，先用合適的中間溶劑清洗色譜柱。應使用至少五個柱體積的中間溶劑。

色譜柱修復程序
按照下面列出的適當修復程序，使用1.0或2.0 mL / min的流速。除非另有規定。使用每列隨附的性能測試報告中列出的測試混合物和條件評估色譜柱性能。

柱修復程序

Packing Material Name	Restoration Procedure
PRP-C18	Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.
PRP-1	Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.
PRP-3	Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.
PRP-h5	Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.
HxSil C8	Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.
HxSil C18	Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.
PRP-X100	Pump approximay 50 mL of methanol with 1% 6 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.
PRP-X200	Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.
PRP-X300	Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.
PRP-X400	Inject Several times with 100 µL of 0.1 M potassium EDTA. Equilibrate with starting phase for ~ 200 column volumes.
RCX-10	Flush with 50 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes.
RCX-30	Flush with 150 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes.
HC-40	Ca2+ Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.
HC-75	Ca2+ Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.
	H+ Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.
	Pb2+ Flush with 1% lead nitrate at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

PRP-X100色譜柱

零件號/ REF	描述
79641	分析型HPLC色譜柱，CUSTOM ORDER（1.0,2.1,4.1,4.6 mm ID）
79669	負離子快
79670	負離子微
79668	負離子分辨率
79643	制備型HPLC色譜柱＃1，定制（21.2,30 mm內徑）
79644	制備型HPLC色譜柱＃2，定制（50 mm內徑）
79645	制備型HPLC色譜柱＃3，CUSTOM ORDER（100 mm ID）
79421	PRP-X10010μm2.1x 150 mm
79346	PRP-X10010μm2.1x 250 mm
79439	PRP-X10010μm4.1x 100 mm
79434	PRP-X10010μm4.1x 150 mm
79433	PRP-X10010μm4.1x 250 mm
79365	PRP-X10010μm4.1x 50 mm
79354	PRP-X10010μm4.6x 150 mm PEEK
79455	PRP-X10010μm4.6x 250 mm PEEK
79353	PRP-X10012-20μm100Å21.2x 250 mm
79852	PRP-X1005μm2.1x 150 mm PEEK
79190	PRP-X1005μm2.1x 250 mm
79538	PRP-X1005μm4.1x 100 mm
79812	PRP-X1005μm4.1x 150 mm
79810	PRP-X1005μm4.1x 50 mm
79174	PRP-X1005μm4.6x 150 mm PEEK
79181	PRP-X1005μm4.6x 250 mm PEEK
79665	PRP-X1007μm4.6x 150 mm PEEK
79642	半制備型HPLC色譜柱，CUSTOM ORDER（7.0,7.8,10 mm ID）

貨號	品名
79477	Analytical Guard Cartridge Holder, PEEK
32908	Analytical Guard Cartridge Holder, Stainless Steel
79293	Analytical Guard Column, PRP-X110
79641	Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID)
79643	Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID)
79644	Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID)
79645	Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID)
79732	PRP-X110 7 µm 4.1 x 150 mm
79734	PRP-X110 7 µm 4.1 x 250 mm
79738	PRP-X110 7 µm 4.6 x 150 mm PEEK
79729	PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), PEEK
79728	PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel
79727	PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK
79726	PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel
79827	PRP-X110, 7 µm Bulk Resin
79743	PRP-X110S 7 µm 2.1 x 100 mm PEEK
79733	PRP-X110S 7 µm 4.1 x 150 mm
79735	PRP-X110S 7 µm 4.1 x 250 mm
79741	PRP-X110S 7 µm 4.6 x 250 mm PEEK
79642	Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID)

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